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2019


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Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity

Alarcon-Correa, M., Guenther, J., Troll, J., Kadiri, V. M., Bill, J., Fischer, P., Rothenstein, D.

ACS Nano, March 2019 (article)

Abstract
Catalytically active colloids are model systems for chemical motors and active matter. It is desirable to replace the inorganic catalysts and the toxic fuels that are often used, with biocompatible enzymatic reactions. However, compared to inorganic catalysts, enzyme-coated colloids tend to exhibit less activity. Here, we show that the self-assembly of genetically engineered M13 bacteriophages that bind enzymes to magnetic beads ensures high and localized enzymatic activity. These phage-decorated colloids provide a proteinaceous environment for directed enzyme immobilization. The magnetic properties of the colloidal carrier particle permit repeated enzyme recovery from a reaction solution, while the enzymatic activity is retained. Moreover, localizing the phage-based construct with a magnetic field in a microcontainer allows the enzyme-phage-colloids to function as an enzymatic micropump, where the enzymatic reaction generates a fluid flow. This system shows the fastest fluid flow reported to date by a biocompatible enzymatic micropump. In addition, it is functional in complex media including blood where the enzyme driven micropump can be powered at the physiological blood-urea concentration.

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link (url) DOI [BibTex]


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Absolute diffusion measurements of active enzyme solutions by NMR

Guenther, J., Majer, G., Fischer, P.

J. Chem. Phys., 150(124201), March 2019 (article)

Abstract
The diffusion of enzymes is of fundamental importance for many biochemical processes. Enhanced or directed enzyme diffusion can alter the accessibility of substrates and the organization of enzymes within cells. Several studies based on fluorescence correlation spectroscopy (FCS) report enhanced diffusion of enzymes upon interaction with their substrate or inhibitor. In this context, major importance is given to the enzyme fructose-bisphosphate aldolase, for which enhanced diffusion has been reported even though the catalysed reaction is endothermic. Additionally, enhanced diffusion of tracer particles surrounding the active aldolase enzymes has been reported. These studies suggest that active enzymes can act as chemical motors that self-propel and give rise to enhanced diffusion. However, fluorescence studies of enzymes can, despite several advantages, suffer from artefacts. Here we show that the absolute diffusion coefficients of active enzyme solutions can be determined with Pulsed Field Gradient Nuclear Magnetic Resonance (PFG-NMR). The advantage of PFG-NMR is that the motion of the molecule of interest is directly observed in its native state without the need for any labelling. Further, PFG-NMR is model-free and thus yields absolute diffusion constants. Our PFG-NMR experiments of solutions containing active fructose-bisphosphate aldolase from rabbit muscle do not show any diffusion enhancement for the active enzymes nor the surrounding molecules. Additionally, we do not observe any diffusion enhancement of aldolase in the presence of its inhibitor pyrophosphate.

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link (url) DOI [BibTex]


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Chemical Nanomotors at the Gram Scale Form a Dense Active Optorheological Medium

Choudhury, U., Singh, D. P., Qiu, T., Fischer, P.

Adv. Mat., (1807382), Febuary 2019 (article)

Abstract
The rheological properties of a colloidal suspension are a function of the concentration of the colloids and their interactions. While suspensions of passive colloids are well studied and have been shown to form crystals, gels, and glasses, examples of energy‐consuming “active” colloidal suspensions are still largely unexplored. Active suspensions of biological matter, such as motile bacteria or dense mixtures of active actin–motor–protein mixtures have, respectively, reveals superfluid‐like and gel‐like states. Attractive inanimate systems for active matter are chemically self‐propelled particles. It has so far been challenging to use these swimming particles at high enough densities to affect the bulk material properties of the suspension. Here, it is shown that light‐triggered asymmetric titanium dioxide that self‐propel, can be obtained in large quantities, and self‐organize to make a gram‐scale active medium. The suspension shows an activity‐dependent tenfold reversible change in its bulk viscosity.

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link (url) DOI [BibTex]


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First Observation of Optical Activity in Hyper-Rayleigh Scattering

Collins, J., Rusimova, K., Hooper, D., Jeong, H. H., Ohnoutek, L., Pradaux-Caggiano, F., Verbiest, T., Carbery, D., Fischer, P., Valev, V.

Phys. Rev. X, 9(011024), January 2019 (article)

Abstract
Chiral nano- or metamaterials and surfaces enable striking photonic properties, such as negative refractive index and superchiral light, driving promising applications in novel optical components, nanorobotics, and enhanced chiral molecular interactions with light. In characterizing chirality, although nonlinear chiroptical techniques are typically much more sensitive than their linear optical counterparts, separating true chirality from anisotropy is a major challenge. Here, we report the first observation of optical activity in second-harmonic hyper-Rayleigh scattering (HRS). We demonstrate the effect in a 3D isotropic suspension of Ag nanohelices in water. The effect is 5 orders of magnitude stronger than linear optical activity and is well pronounced above the multiphoton luminescence background. Because of its sensitivity, isotropic environment, and straightforward experimental geometry, HRS optical activity constitutes a fundamental experimental breakthrough in chiral photonics for media including nanomaterials, metamaterials, and chemical molecules.

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link (url) DOI [BibTex]

link (url) DOI [BibTex]


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Microrobotics and Microorganisms: Biohybrid Autonomous Cellular Robots

Alapan, Y., Yasa, O., Yigit, B., Yasa, I. C., Erkoc, P., Sitti, M.

Annual Review of Control, Robotics, and Autonomous Systems, 2019 (article)

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X-ray Optics Fabrication Using Unorthodox Approaches

Sanli, U., Baluktsian, M., Ceylan, H., Sitti, M., Weigand, M., Schütz, G., Keskinbora, K.

Bulletin of the American Physical Society, APS, 2019 (article)

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The near and far of a pair of magnetic capillary disks

Koens, L., Wang, W., Sitti, M., Lauga, E.

Soft Matter, 2019 (article)

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Review of emerging concepts in nanotoxicology: opportunities and challenges for safer nanomaterial design

Singh, A. V., Laux, P., Luch, A., Sudrik, C., Wiehr, S., Wild, A., Santamauro, G., Bill, J., Sitti, M.

Toxicology Mechanisms and Methods, 2019 (article)

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Graphene oxide synergistically enhances antibiotic efficacy in Vancomycin resistance Staphylococcus aureus

Singh, V., Kumar, V., Kashyap, S., Singh, A. V., Kishore, V., Sitti, M., Saxena, P. S., Srivastava, A.

ACS Applied Bio Materials, ACS Publications, 2019 (article)

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Microfluidics Integrated Lithography‐Free Nanophotonic Biosensor for the Detection of Small Molecules

Sreekanth, K. V., Sreejith, S., Alapan, Y., Sitti, M., Lim, C. T., Singh, R.

Advanced Optical Materials, 2019 (article)

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Mobile microrobots for active therapeutic delivery

Erkoc, P., Yasa, I. C., Ceylan, H., Yasa, O., Alapan, Y., Sitti, M.

Advanced Therapeutics, Wiley Online Library, 2019 (article)

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Electromechanical actuation of dielectric liquid crystal elastomers for soft robotics

Davidson, Z., Shahsavan, H., Guo, Y., Hines, L., Xia, Y., Yang, S., Sitti, M.

Bulletin of the American Physical Society, APS, 2019 (article)

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2008


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Voltage-Controllable Magnetic Composite Based on Multifunctional Polyethylene Microparticles

Ghosh, A., Sheridon, N. K., Fischer, P.

SMALL, 4(11):1956-1958, 2008 (article)

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DOI [BibTex]

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ENHANCED ADHESION OF PDMS SURFACES FUNCTIONALIZED BY POLY (n-BUTYL ACRYLATE) BRUSHES INSPIRED BY GECKO FOOT HAIRS

Nese, A., Lee, H., Dong, H., Aksak, B., Cusick, B., Kowalewski, T., Matyjaszewski, K., Sitti, M.

Polymer Preprints, 49(2):107, 2008 (article)

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Design and development of the lifting and propulsion mechanism for a biologically inspired water runner robot

Floyd, S., Sitti, M.

IEEE transactions on robotics, 24(3):698-709, IEEE, 2008 (article)

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Control of Cell Behavior by Aligned Micro/Nanofibrous Biomaterial Scaffolds Fabricated by Spinneret-Based Tunable Engineered Parameters (STEP) Technique

Nain, A. S., Phillippi, J. A., Sitti, M., MacKrell, J., Campbell, P. G., Amon, C.

Small, 4(8):1153-1159, Wiley Online Library, 2008 (article)

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Simulation and analysis of a passive pitch reversal flapping wing mechanism for an aerial robotic platform

Arabagi, V., Sitti, M.

In Intelligent Robots and Systems, 2008. IROS 2008. IEEE/RSJ International Conference on, pages: 1260-1265, 2008 (inproceedings)

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Project Page [BibTex]


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Biologically Inspired Polymer Micro-Patterned Adhesives

Cheung, E., Sitti, M.

EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD, 2008 (techreport)

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Rolling and spinning friction characterization of fine particles using lateral force microscopy based contact pushing

Sümer, B., Sitti, M.

Journal of Adhesion Science and Technology, 22(5-6):481-506, Taylor & Francis Group, 2008 (article)

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Fabrication and Characterization of Biologically Inspired Mushroom-Shaped Elastomer Microfiber Arrays

Kim, S., Sitti, M.

In ASME 2008 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, pages: 839-847, 2008 (inproceedings)

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Project Page [BibTex]


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Gecko inspired micro-fibrillar adhesives for wall climbing robots on micro/nanoscale rough surfaces

Aksak, B., Murphy, M. P., Sitti, M.

In Robotics and Automation, 2008. ICRA 2008. IEEE International Conference on, pages: 3058-3063, 2008 (inproceedings)

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Project Page [BibTex]


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Modeling the soft backing layer thickness effect on adhesion of elastic microfiber arrays

Long, R., Hui, C., Kim, S., Sitti, M.

Journal of Applied Physics, 104(4):044301, AIP, 2008 (article)

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Project Page [BibTex]


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Cross-talk compensation in atomic force microscopy

Onal, C. D., Sümer, B., Sitti, M.

Review of scientific instruments, 79(10):103706, AIP, 2008 (article)

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Miniature Mobile Robots Down to Micron Scale

Sitti, M.

In Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on, pages: 525-525, 2008 (inproceedings)

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Adhesion of biologically inspired oil-coated polymer micropillars

Cheung, E., Sitti, M.

Journal of Adhesion Science and Technology, 22(5-6):569-589, Taylor & Francis Group, 2008 (article)

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Polymeric Micro/Nanofiber Manufacturing and Mechanical Characterization

Nain, A. S., Sitti, M., Amon, C.

In ASME 2008 International Mechanical Engineering Congress and Exposition, pages: 295-303, 2008 (inproceedings)

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An untethered magnetically actuated micro-robot capable of motion on arbitrary surfaces

Floyd, S., Pawashe, C., Sitti, M.

In Robotics and Automation, 2008. ICRA 2008. IEEE International Conference on, pages: 419-424, 2008 (inproceedings)

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[BibTex]


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Fabrication of bio-inspired elastomer nanofiber arrays with spatulate tips using notching effect

Kim, S., Sitti, M., Jang, J., Thomas, E. L.

In Nanotechnology, 2008. NANO’08. 8th IEEE Conference on, pages: 780-782, 2008 (inproceedings)

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A motorized anchoring mechanism for a tethered capsule robot using fibrillar adhesives for interventions in the esophagus

Glass, P., Cheung, E., Wang, H., Appasamy, R., Sitti, M.

In Biomedical Robotics and Biomechatronics, 2008. BioRob 2008. 2nd IEEE RAS & EMBS International Conference on, pages: 758-764, 2008 (inproceedings)

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Vision-based feedback strategy for controlled pushing of microparticles

Lynch, N. A., Onal, C. D., Schuster, E., Sitti, M.

Journal of Micro-Nano Mechatronics, 4(1-2):73-83, Springer-Verlag, 2008 (article)

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Effect of quantity and configuration of attached bacteria on bacterial propulsion of microbeads

Behkam, B., Sitti, M.

Applied Physics Letters, 93(22):223901, AIP, 2008 (article)

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Fabrication of Single and Multi-Layer Fibrous Biomaterial Scaffolds for Tissue Engineering

Nain, A. S., Miller, E., Sitti, M., Campbell, P., Amon, C.

In ASME 2008 International Mechanical Engineering Congress and Exposition, pages: 231-238, 2008 (inproceedings)

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Performance of different foot designs for a water running robot

Floyd, S., Adilak, S., Ramirez, S., Rogman, R., Sitti, M.

In Robotics and Automation, 2008. ICRA 2008. IEEE International Conference on, pages: 244-250, 2008 (inproceedings)

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Dynamic modeling of a basilisk lizard inspired quadruped robot running on water

Park, H. S., Floyd, S., Sitti, M.

In Intelligent Robots and Systems, 2008. IROS 2008. IEEE/RSJ International Conference on, pages: 3101-3107, 2008 (inproceedings)

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Bacterial propulsion of chemically patterned micro-cylinders

Behkam, B., Sitti, M.

In Biomedical Robotics and Biomechatronics, 2008. BioRob 2008. 2nd IEEE RAS & EMBS International Conference on, pages: 753-757, 2008 (inproceedings)

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Preface to the Journal of Micro-Nano Mechatronics

Dario, P., Fukuda, T., Sitti, M.

Journal of Micro-Nano Mechatronics, 4(1-2):1-1, Springer-Verlag, 2008 (article)

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A legged anchoring mechanism for capsule endoscopes using micropatterned adhesives

Glass, P., Cheung, E., Sitti, M.

IEEE Transactions on Biomedical Engineering, 55(12):2759-2767, IEEE, 2008 (article)

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Project Page [BibTex]


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Design and Numerical Modeling of an On-Board Chemical Release Module for Motion Control of Bacteria-Propelled Swimming Micro-Robots

Behkam, B., Nain, A. S., Amon, C. H., Sitti, M.

In ASME 2008 International Mechanical Engineering Congress and Exposition, pages: 239-244, 2008 (inproceedings)

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[BibTex]


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Dynamic modeling of stick slip motion in an untethered magnetic microrobot

Pawashe, C., Floyd, S., Sitti, M.

Proceedings of Robotics: Science and Systems IV, Zurich, Switzerland, 2008 (article)

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Investigation of Calcium Mechanotransduction by Quasi 3-D Microfiber Mechanical Stimulation of Cells

Ruder, W. C., Pratt, E. D., Sitti, M., LeDuc, P. R., Antaki, J. F.

In ASME 2008 Summer Bioengineering Conference, pages: 1049-1050, 2008 (inproceedings)

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[BibTex]


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Beanbag robotics: Robotic swarms with 1-dof units

Kriesel, D. M., Cheung, E., Sitti, M., Lipson, H.

In International Conference on Ant Colony Optimization and Swarm Intelligence, pages: 267-274, 2008 (inproceedings)

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[BibTex]


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Particle image velocimetry and thrust of flagellar micro propulsion systems

Danis, U., Sitti, M., Pekkan, K.

In APS Division of Fluid Dynamics Meeting Abstracts, 1, 2008 (inproceedings)

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